Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners

AHCSDT303 Mapping and Delivery Guide
Perform a fluorescence test on seeds

Version 1.0
Issue Date: May 2024


Qualification -
Unit of Competency AHCSDT303 - Perform a fluorescence test on seeds
Description
Employability Skills
Learning Outcomes and Application This unit of competency describes the skills and knowledge required to perform tests that determine the presence of seeds of annual ryegrass species in seedlots of perennial ryegrass. It applies to seed analysts working within enterprise requirements and procedures.It applies to individuals who take responsibility for their own work and for the quality of the work of others. They use discretion and judgement in the selection, allocation and use of available resources. All work is carried out to comply with workplace procedures.No occupational licensing, legislative or certification requirements are known to apply to this unit at the time of publication.
Duration and Setting X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting.

Assessors must satisfy current standards for RTOs.
Prerequisites/co-requisites
Competency Field
Development and validation strategy and guide for assessors and learners Student Learning Resources Handouts
Activities 		Slides
PPT 		Assessment 1 Assessment 2 Assessment 3 Assessment 4
Elements of Competency Performance Criteria              
Element: Prepare for a fluorescence test
  • Identify seed to be tested and complete records
  • Prepare working sample from submitted sample
  • Prepare the required number of replicates of 100 seeds
  • Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary
  • Place replicates in a germination cabinet/room
       
Element: Assess germinated seeds
  • Count off and record all un-germinated seeds and abnormal seedlings
  • Examine normal seedlings under ultra violet light
  • Count off and record normal seedlings that have fluorescent roots under ultra violet light
  • Count off and record normal seedlings that do not have fluorescent roots under ultra violet light
  • Minimise exposure of bare skin to ultra violet light
  • Complete enterprise worksheet
       
Element: Maintain a safe work environment
  • Record, calculate and report results of test
  • Collect and dispose of laboratory and hazardous waste safely
  • Clean and store apparatus and equipment
       


Evidence Required

List the assessment methods to be used and the context and resources required for assessment. Copy and paste the relevant sections from the evidence guide below and then re-write these in plain English.

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare for a fluorescence test

1.1 Identify seed to be tested and complete records

1.2 Prepare working sample from submitted sample

1.3 Prepare the required number of replicates of 100 seeds

1.4 Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary

1.5 Place replicates in a germination cabinet/room

2. Assess germinated seeds

2.1 Count off and record all un-germinated seeds and abnormal seedlings

2.2 Examine normal seedlings under ultra violet light

2.3 Count off and record normal seedlings that have fluorescent roots under ultra violet light

2.4 Count off and record normal seedlings that do not have fluorescent roots under ultra violet light

2.5 Minimise exposure of bare skin to ultra violet light

2.6 Complete enterprise worksheet

3. Maintain a safe work environment

3.1 Record, calculate and report results of test

3.2 Collect and dispose of laboratory and hazardous waste safely

3.3 Clean and store apparatus and equipment

The candidate must be assessed on their ability to integrate and apply the performance requirements of this unit in a workplace setting. Performance must be demonstrated consistently over time and in a suitable range of contexts.

The candidate must provide evidence that they can:

prepare for a fluorescence test

assess seedlings using ultra violet light

count and record test results

prepare replicates from working sample

test to determine the presence of seeds of annual ryegrass species in perennial ryegrass seedlots

maintain a safe work environment

The candidate must demonstrate knowledge of:

enterprise and/or legal traceability requirements

enterprise standard operating procedures (SOPs)

fluorescence testing procedures

relevant work health, safety and environment requirements

seed biology

the role of the International Seed Testing Association (ISTA) and the ISTA Rules for Seed Testing

Submission Requirements

List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here

Assessment task 1: [title]      Due date:

(add new lines for each of the assessment tasks)

Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.

Element

Performance criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1. Prepare for a fluorescence test

1.1 Identify seed to be tested and complete records

1.2 Prepare working sample from submitted sample

1.3 Prepare the required number of replicates of 100 seeds

1.4 Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary

1.5 Place replicates in a germination cabinet/room

2. Assess germinated seeds

2.1 Count off and record all un-germinated seeds and abnormal seedlings

2.2 Examine normal seedlings under ultra violet light

2.3 Count off and record normal seedlings that have fluorescent roots under ultra violet light

2.4 Count off and record normal seedlings that do not have fluorescent roots under ultra violet light

2.5 Minimise exposure of bare skin to ultra violet light

2.6 Complete enterprise worksheet

3. Maintain a safe work environment

3.1 Record, calculate and report results of test

3.2 Collect and dispose of laboratory and hazardous waste safely

3.3 Clean and store apparatus and equipment

Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Identify seed to be tested and complete records 
Prepare working sample from submitted sample 
Prepare the required number of replicates of 100 seeds 
Prepare substrate as prescribed for a germination test using dormancy breaking treatments if necessary 
Place replicates in a germination cabinet/room 
Count off and record all un-germinated seeds and abnormal seedlings 
Examine normal seedlings under ultra violet light 
Count off and record normal seedlings that have fluorescent roots under ultra violet light 
Count off and record normal seedlings that do not have fluorescent roots under ultra violet light 
Minimise exposure of bare skin to ultra violet light 
Complete enterprise worksheet 
Record, calculate and report results of test 
Collect and dispose of laboratory and hazardous waste safely 
Clean and store apparatus and equipment 

Forms

Assessment Cover Sheet

AHCSDT303 - Perform a fluorescence test on seeds
Assessment task 1: [title]

Student name:

Student ID:

I declare that the assessment tasks submitted for this unit are my own work.

Student signature:

Result: Competent Not yet competent

Feedback to student

 

 

 

 

 

 

 

 

Assessor name:

Signature:

Date:

Assessment Record Sheet

AHCSDT303 - Perform a fluorescence test on seeds

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

(add lines for each task)

Feedback to student:

 

 

 

 

 

 

 

 

Overall assessment result: Competent Not yet competent

Assessor name:

Signature:

Date:

Student signature:

Date: